Plant mitochondria and chloroplasts are targeted by the Rhizoctonia solani RsCRP1 effector

The fungal species Rhizoctonia solani belongs to the Basidiomycota division and is a ubiquitous soil-borne pathogen. It main agent of damping-off disease in seedlings causes root crown rot sugar beets. Plant pathogens deploy small secreted proteins, called effectors, manipulate plant immunity order infect host. Here, gene (RsCRP1) encoded putative effector cysteine-rich protein was cloned, expressed Cercospora beticola used for virulence assays. RsCRP1 highly induced upon early-infection stage beet promoted. Confocal microscopy demonstrated localization chloroplasts mitochondria transient expression leaves Nicotiana benthamiana. Further, this unable induce necrosis or suppress hypersensitive response by Avr4/Cf4 complex N. Overall, these data indicate that novel targeting distinct cell organelles facilitate successful infection at early stages development.